Coding
creA

Part:BBa_K5108003:Design

Designed by: Léa Breton   Group: iGEM24_Toulouse-INSA-UPS   (2024-08-28)


Creatinase from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI site found at 1012


Sources

The codon-optimized nucleotide sequence from Pseudomonas putida adapted to Pseudomonas putida was obtained using the Codon Optimizer Tool from Integrated DNA Technologies. This step permits optimize codon usage, lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis.

References

  • UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry
  • Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/